Recombinant human AMELX protein
AMELX is a member of the amelogenin family of extracellular matrix proteins. Amelogenins are involved in biomineralization during tooth enamel development. Mutations in AMELX cause X-linked amelogenesis imperfecta. Alternative splicing results in multiple transcript variants encoding different isoforms. Recombinant human AMELX protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
22.3 kDa (198aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Patir A, Seymen F, et al. (2008) . Caries Res. 42 (5) :394-400.; ; Zhu L, Tanimoto K, et al. (2009) . Cells Tissues Organs. 189 (1-4) :203-6.;
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 30% glycerol, 1mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
NP_001133
Uniprot Accession Number
Q99217
Species
Human
AA Sequence
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
