Recombinant human BCL2L10 protein

BCL2L10 belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BCL2L10 contains conserved BH4, BH1 and BH2 domains. This protein can interact with other members of BCL-2 protein family including BCL2, BCL2L1/BCL-X (L), and BAX. Overexpression of this protein has been shown to suppress cell apoptosis possibly through the prevention of cytochrome C release from the mitochondria, and thus activating caspase-3 activation. Recombinant human BCL2L10 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Bcl-2-like protein 10, BCL-B, Boo, Diva

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

21.8 kDa (195aa) confirmed by MALDI-TOF

Additionnal Information

BCL2L10, Bcl-2-like protein 10, BCL-B, Boo, Diva, ATGP1857-10 µg, ATGP1857-20 µg, ATGP1857-50 µg, ATGP1857-100 µg, ATGP1857-250 µg, ATGP1857-500 µg, ATGP1857-1 mg, ATGP1857-10, ATGP1857-20, ATGP1857-50, ATGP1857-100, ATGP1857-250, ATGP1857-500, ATGP1857-1

References & Citations

Kang Y, Lee DC, et al. (2007) . Biochem Biophys Res Commun. 359 (1) :76-82.; ; Guillemin Y, Lalle P, et al. (2009) . J Mol Med (Berl) . 87 (9) :923-40.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl,20% glycerol, 1mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_065129

Uniprot Accession Number

Q9HD36

Species

Human

AA Sequence

MVDQLRE RTTMADPLRE RTELLLADYL GYCAREPGTP EPAPSTPEAA VLRSAAARLR QIHRSFFSAY LGYPGNRFEL VALMADSVLS DSPGPTWGRV VTLVTFAGTL LERGPLVTAR WKKWGFQPRL KEQEGDVARD CQRLVALLSS RLMGQHRAWL QAQGGWDGFC HFFRT