Recombinant human UFSP1 protein
UFSP1 is similar to other ufm1-specific proteases. Studies in mouse determined that ufsp1 releases ufm1 (ubiquitin-fold modifier 1) from its bound conjugated complexes which also makes it into an active form. Because the human uFSP1 protein is shorter on the N-terminus and lacks a conserved Cys active site, it is predicted to be non-functional. Recombinant human uFSP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Inactive ufm1-specific protease 1, uFSP
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
17.4 kDa (165aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Wilson MD, Riemer C, et al. (2001) . Nucleic Acids Res. 29 (6) :1352-65
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 30% glycerol, 1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_001015072
Uniprot Accession Number
Q6NVU6
Species
Human
AA Sequence
Frequently Asked Questions
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