Recombinant human MED20 protein

MED20 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II (see MIM 180660), and general initiation factors. Recombinant human MED20 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Mediator of RNA polymerase II transcription subunit 20, PRO0213, TRFP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

25.6 kDa (235aa) confirmed by MALDI-TOF

Additionnal Information

MED20, Mediator of RNA polymerase II transcription subunit 20, PRO0213, TRFP, ATGP1855-10 µg, ATGP1855-20 µg, ATGP1855-50 µg, ATGP1855-100 µg, ATGP1855-250 µg, ATGP1855-500 µg, ATGP1855-1 mg, ATGP1855-10, ATGP1855-20, ATGP1855-50, ATGP1855-100, ATGP1855-250, ATGP1855-500, ATGP1855-1

References & Citations

Sato S, Tomomori-Sato C, et al. (2004) . Mol Cell. 14 (5) :685-91.; ; Zhang X, Krutchinsky A, et al. (2011) . Mol Cell. 19 (1) :89-100.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 50% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_004266

Uniprot Accession Number

Q9H944

Species

Human

AA Sequence

MGVTCVS QMPVAEGKSV QQTVELLTRK LEMLGAEKQG TFCVDCETYH TAASTLGSQG QTGKLMYVMH NSEYPLSCFA LFENGPCLIA DTNFDVLMVK LKGFFQSAKA SKIETRGTRY QYCDFLVKVG TVTMGPSARG ISVEVEYGPC VVASDCWSLL LEFLQSFLGS HTPGAPAVFG NRHDAVYGPA DTMVQYMELF NKIRKQQQVP VAGIR