Recombinant human MED20 protein
MED20 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II (see MIM 180660), and general initiation factors. Recombinant human MED20 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Mediator of RNA polymerase II transcription subunit 20, PRO0213, TRFP
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
25.6 kDa (235aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Sato S, Tomomori-Sato C, et al. (2004) . Mol Cell. 14 (5) :685-91.; ; Zhang X, Krutchinsky A, et al. (2011) . Mol Cell. 19 (1) :89-100.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 50% glycerol, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_004266
Uniprot Accession Number
Q9H944
Species
Human
AA Sequence
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