Recombinant human BCL7A protein

BCL7A is directly involved, with Myc and IgH, in a three-way gene translocation in a Burkitt lymphoma cell line. As a result of the gene translocation, the N-terminal region of the gene is disrupted, which is thought to be related to the pathogenesis of a subset of high-grade B cell non-Hodgkin lymphoma. The N-terminal segment involved in the translocation includes the region that shares a strong sequence similarity with those of BCL7B and BCL7C. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human BCL7A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

B-cell CLL/lymphoma 7 protein family member A, BCL7

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

25.2 kDa (233aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

BCL7A, B-cell CLL/lymphoma 7 protein family member A, BCL7, ATGP1854-10 µg, ATGP1854-20 µg, ATGP1854-50 µg, ATGP1854-100 µg, ATGP1854-250 µg, ATGP1854-500 µg, ATGP1854-1 mg, ATGP1854-10, ATGP1854-20, ATGP1854-50, ATGP1854-100, ATGP1854-250, ATGP1854-500, ATGP1854-1

References & Citations

Carbone, A., et al. (2008) Genes Chromosomes Cancer 47 (12), 1067-1075; ; Morton, L.M., et al. (2009) Cancer Epidemiol. Biomarkers Prev. 18 (4), 1259-1270;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT, 2mM EDTA

Scientific Category

Metabolism

NCBI Accession Number

NP_001019979

Uniprot Accession Number

Q4VC05

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSGRSVR AETRSRAKDD IKRVMAAIEK VRKWEKKWVT VGDTSLRIYK WVPVTEPKVD DKNKNKKKGK DEKCGSEVTT PENSSSPGMM DMHDDNSNQS SIADASPIKQ ENSSNSSPAP EPNSAVPSDG TEAKVDEAQA DGKEHPGAED ASDEQNSQSS MEHSMNSSEK VDRQPSGDSG LAAETSAISQ DLEGVPPSKK MKLEASQQNS EEM