Recombinant human TYW5 protein
TRNA wybutosine-synthesizing protein 5, also known as TYW5, acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. TYW5 catalyzes the hydroxylation of 7- (a-amino-a-carboxypropyl) wyosine (yW-72) into undermodified hydroxywybutosine (OHyW) . OHyW is a derivative of wybutosine found in higher eukaryotes. Recombinant human TYW5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
TRNA wybutosine-synthesizing protein 5, C2orf60, Htyw5
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
38.9 kDa (338aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Noma A., et al. (2010) J. Biol. Chem. 285:34503-34507; ; Kato M., et al. (2011) Nucleic Acids Res. 39:1576-1585;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_001034782
Uniprot Accession Number
A2RUC4
Species
Human
AA Sequence
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