Recombinant human TYW5 protein

TRNA wybutosine-synthesizing protein 5, also known as TYW5, acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. TYW5 catalyzes the hydroxylation of 7- (a-amino-a-carboxypropyl) wyosine (yW-72) into undermodified hydroxywybutosine (OHyW) . OHyW is a derivative of wybutosine found in higher eukaryotes. Recombinant human TYW5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

TRNA wybutosine-synthesizing protein 5, C2orf60, Htyw5

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

38.9 kDa (338aa) confirmed by MALDI-TOF

Additionnal Information

TYW5, tRNA wybutosine-synthesizing protein 5, C2orf60, Htyw5, ATGP1851-10 µg, ATGP1851-20 µg, ATGP1851-50 µg, ATGP1851-100 µg, ATGP1851-250 µg, ATGP1851-500 µg, ATGP1851-1 mg, ATGP1851-10, ATGP1851-20, ATGP1851-50, ATGP1851-100, ATGP1851-250, ATGP1851-500, ATGP1851-1

References & Citations

Noma A., et al. (2010) J. Biol. Chem. 285:34503-34507; ; Kato M., et al. (2011) Nucleic Acids Res. 39:1576-1585;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001034782

Uniprot Accession Number

A2RUC4

Species

Human

AA Sequence

MAGQHLP VPRLEGVSRE QFMQHLYPQR KPLVLEGIDL GPCTSKWTVD YLSQVGGKKE VKIHVAAVAQ MDFISKNFVY RTLPFDQLVQ RAAEEKHKEF FVSEDEKYYL RSLGEDPRKD VADIRKQFPL LKGDIKFPEF FKEEQFFSSV FRISSPGLQL WTHYDVMDNL LIQVTGKKRV VLFSPRDAQY LYLKGTKSEV LNIDNPDLAK YPLFSKARRY ECSLEAGDVL FIPALWFHNV ISEEFGVGVN IFWKHLPSEC YDKTDTYGNK DPTAASRAAQ ILDRALKTLA ELPEEYRDFY ARRMVLHIQD KAYSKNSE