Recombinant human SMUG1 protein
Single-strand-selective monofunctional uracil-DNA glycosylase, also known as SMuG1, is a enzyme responsible for recognizing base lesions in the genome and initiating base excision DNA repair. This protein acts as a monofunctional DNA glycosylase specific for uracil (u) residues in DNA and has a preference for single-stranded DNA substrates. Recombinant human SMuG1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Single-strand-selective monofunctional uracil-DNA glycosylase, FDG, HMuDG, uNG3
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
32.3 kDa (293aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Masaoka A., et al. (2003) Biochemistry. 42:5003-5012; ; Boorstein R.J., et al. (2001) J. Biol. Chem. 276:41991-41997;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 30% glycerol, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_055126
Uniprot Accession Number
Q53HV7
Species
Human
AA Sequence
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
