Recombinant human RWDD1 protein
RWDD1, also known as CGI-24 or PTD013, belongs to the RWDD1/GIR2 family. This protein protects DRG2 (developmentally regulated GTP binding protein 2), from proteolytic degradation. RWDD1 is a novel RWD domain containing protein. RWD domain refers to three signature motifs in proteins: RING finger, WD-repeats, and yeast DEAD (DEXD) -like motif. The function of this domain is not clear enough. Present evidence suggests that RWD domain might be necessary for protein-protein interaction. Recombinant human RWDD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
RWD domain-containing protein 1, CGI-24, PTD013
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
30.3 kDa (266aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Kang N, et al. (2008) Cell Mol Immunol. 5 (5) :333-9.; ; Grotsch H, et al. (2012) Mol Cell Endocrinol. 358 (1) :53-62.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl,10% glycerol, 2mM DTT
Scientific Category
Protease inhibitors
NCBI Accession Number
NP_057036
Uniprot Accession Number
Q9H446
Species
Human
AA Sequence
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