Recombinant human RWDD1 protein

RWDD1, also known as CGI-24 or PTD013, belongs to the RWDD1/GIR2 family. This protein protects DRG2 (developmentally regulated GTP binding protein 2), from proteolytic degradation. RWDD1 is a novel RWD domain containing protein. RWD domain refers to three signature motifs in proteins: RING finger, WD-repeats, and yeast DEAD (DEXD) -like motif. The function of this domain is not clear enough. Present evidence suggests that RWD domain might be necessary for protein-protein interaction. Recombinant human RWDD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

RWD domain-containing protein 1, CGI-24, PTD013

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

30.3 kDa (266aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

RWDD1, RWD domain-containing protein 1, CGI-24, PTD013, ATGP1844-10 µg, ATGP1844-20 µg, ATGP1844-50 µg, ATGP1844-100 µg, ATGP1844-250 µg, ATGP1844-500 µg, ATGP1844-1 mg, ATGP1844-10, ATGP1844-20, ATGP1844-50, ATGP1844-100, ATGP1844-250, ATGP1844-500, ATGP1844-1

References & Citations

Kang N, et al. (2008) Cell Mol Immunol. 5 (5) :333-9.; ; Grotsch H, et al. (2012) Mol Cell Endocrinol. 358 (1) :53-62.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl,10% glycerol, 2mM DTT

Scientific Category

Protease inhibitors

NCBI Accession Number

NP_057036

Uniprot Accession Number

Q9H446

Species

Human

AA Sequence

MTDYGEE QRNELEALES IYPDSFTVLS ENPPSFTITV TSEAGENDET VQTTLKFTYS EKYPDEAPLY EIFSQENLED NDVSDILKLL ALQAEENLGM VMIFTLVTAV QEKLNEIVDQ IKTRREEEKK QKEKEAEEAE KQLFHGTPVT IENFLNWKAK FDAELLEIKK KRMKEEEQAG KNKLSGKQLF ETDHNLDTSD IQFLEDAGNN VEVDESLFQE MDDLELEDDE DDPDYNPADP ESDSAD