Recombinant human CHMP1A protein

Charged multivesicular body protein 1a, also known as CHMP1A, belongs to the vacuolar sorting protein family and functions as chromatin-modifying protein. These complexes are crucial for sorting endosomal articles into multivesicular bodies (MVBs), as well as required for the formation of these bodies. The MVBs pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. CHMP1 interacts with VPS4B and localizes to early endosomes. Two isoforms, encoded by distinct genes, exists for CHMP1. They are designated CHMP1A and CHMP1B. Recombinant human CHMP1A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Charged multivesicular body protein 1a, CHMP1, PCOLN3, PRSM1, VPS46-1, VPS46A, Chromatin-modifying protein 1a, CHMP1a, Vacuolar protein sorting-associated protein 46-1, hVps46-1, Procollagen type III N-endopeptidase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

24.1 kDa (219aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

CHMP1A, Charged multivesicular body protein 1a, CHMP1, PCOLN3, PRSM1, VPS46-1, VPS46A, ATGP1841-10 µg, ATGP1841-20 µg, ATGP1841-50 µg, ATGP1841-100 µg, ATGP1841-250 µg, ATGP1841-500 µg, ATGP1841-1 mg, ATGP1841-10, ATGP1841-20, ATGP1841-50, ATGP1841-100, ATGP1841-250, ATGP1841-500, ATGP1841-1

References & Citations

Stauffer D R., et al. (2001) J Cell Sci. 114:2383-2393; ; Howard T L., et al. (2001) J Cell Sci. 114: 2395-2404.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol,1mM DTT

Scientific Category

Cell Cycle

NCBI Accession Number

NP_002759.2

Uniprot Accession Number

Q9HD42

Species

Human

AA Sequence

MDDTLFQ LKFTAKQLEK LAKKAEKDSK AEQAKVKKAL LQKNVECARV YAENAIRKKN EGVNWLRMAS RVDAVASKVQ TAVTMKGVTK NMAQVTKALD KALSTMDLQK VSSVMDRFEQ QVQNLDVHTS VMEDSMSSAT TLTTPQEQVD SLIMQIAEEN GLEVLDQLSQ LPEGASAVGE SSVRSQEDQL SRRLAALRN