Recombinant human PARD6B protein

Partitioning defective 6 homolog beta, also known as PARD6B, is a member of the PAR6 family and encodes a protein with a PSD95/Discs-large/ZO1 (PDZ) domain, an OPR domain and a semi-Cdc42/Rac interactive binding (CRIB) domain. Cellular asymmetry is critical for the development of multicellular organisms. PARD (partitioning-defective) proteins play important roles in asymmetric cell division and polarized growth, whereas Cdc42 and Rac mediate establishment of cell growth and polarity and contribute to oncogenic transformation by Ras. It is expressed in pancreas and in both adult and fetal kidney, and is weakly expressed in placenta and lung. Recombinant human PARD6B protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Partitioning defective 6 homolog beta, PAR6B

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

43.6 kDa (395aa)

Additionnal Information

PARD6B, Partitioning defective 6 homolog beta, PAR6B, ATGP1840-10 µg, ATGP1840-20 µg, ATGP1840-50 µg, ATGP1840-100 µg, ATGP1840-250 µg, ATGP1840-500 µg, ATGP1840-1 mg, ATGP1840-10, ATGP1840-20, ATGP1840-50, ATGP1840-100, ATGP1840-250, ATGP1840-500, ATGP1840-1

References & Citations

Joberty G., et al. (2000) Nat Cell Biol. 2:531-539.; ; Qiu R G., et al. (2000) Curr Biol. 10:697-707.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2M urea, 20% glycerol, 0.2M NaCl

Scientific Category

Cell Cycle

NCBI Accession Number

NP_115910

Uniprot Accession Number

Q9BYG5

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMNRSHRH GAGSGCLGTM EVKSKFGAEF RRFSLERSKP GKFEEFYGLL QHVHKIPNVD VLVGYADIHG DLLPINNDDN YHKAVSTANP LLRIFIQKKE EADYSAFGTD TLIKKKNVLT NVLRPDNHRK KPHIVISMPQ DFRPVSSIID VDILPETHRR VRLYKYGTEK PLGFYIRDGS SVRVTPHGLE KVPGIFISRL VPGGLAQSTG LLAVNDEVLE VNGIEVSGKS LDQVTDMMIA NSRNLIITVR PANQRNNVVR NSRTSGSSGQ STDNSLLGYP QQIEPSFEPE DEDSEEDDII IEDNGVPQQI PKAVPNTESL ESLTQIELSF ESGQNGFIPS NEVSLAAIAS SSNTEFETHA PDQKLLEEDG TIITL