Recombinant human PGPEP-1 protein
PGPEP1 is an omega peptidase which removes pyroglutamyl residues from the amino termini of peptides and proteins. It is a cytosolic cysteine peptidase that is expressed in most cell types. The enzyme requires a thiolreducing agent for activity. PGPEP1 is potentially involved in the inactivation of biologically active peptides that possess an amino terminal pyroglutamyl group. Examples of such peptides include neurotensin, luteinizing hormone releasing hormone, and thyrotropinreleasing hormone. Recombinant human PGPEP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Pyroglutamyl-peptidase 1, Pyroglutamyl-peptidase I, Pcp, PGP, PGP-I, PGPI
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
25.5 kDa (232aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kilbane Z. et al. (2007) Mol. Cell. Biochem. 297:189.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_060182
Uniprot Accession Number
Q9NXJ5
Species
Human
AA Sequence
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