Recombinant human CDK5 protein

CDK5, also known as cell division protein kinase 5, is a member of the cyclin-dependent kinase family. It is required for proper development of the brain and to be activated, CDK5 must associate with CDK5R1 or CDK5R2. unlike other cyclin dependent kinases, it does not also require phosphorylation on the T loop so that binding with the activator is sufficient to activate the kinase. This protein is involved in the processes of neuronal maturation and migration, phosphorylating the key intracellular adaptor of the reelin signaling chain. Recombinant human CDK5 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Cyclin-dependent kinase 5, PSSALRE

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

35.8 kDa (316aa)

Additionnal Information

CDK5, Cyclin-dependent kinase 5, PSSALRE, ATGP1835-10 µg, ATGP1835-20 µg, ATGP1835-50 µg, ATGP1835-100 µg, ATGP1835-250 µg, ATGP1835-500 µg, ATGP1835-1 mg, ATGP1835-10, ATGP1835-20, ATGP1835-50, ATGP1835-100, ATGP1835-250, ATGP1835-500, ATGP1835-1

References & Citations

Demetrick DJ. et al. (1994) Cytogenet Cell Genet. 66:72-74.; ; Meyerson M. et al. (1992) EMBO J. 11:2909-2917.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea

Scientific Category

Metabolism

NCBI Accession Number

NP_004926

Uniprot Accession Number

Q00535

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMQKYEK LEKIGEGTYG TVFKAKNRET HEIVALKRVR LDDDDEGVPS SALREICLLK ELKHKNIVRL HDVLHSDKKL TLVFEFCDQD LKKYFDSCNG DLDPEIVKSF LFQLLKGLGF CHSRNVLHRD LKPQNLLINR NGELKLADFG LARAFGIPVR CYSAEVVTLW YRPPDVLFGA KLYSTSIDMW SAGCIFAELA NAGRPLFPGN DVDDQLKRIF RLLGTPTEEQ WPSMTKLPDY KPYPMYPATT SLVNVVPKLN ATGRDLLQNL LKCNPVQRIS AEEALQHPYF SDFCPP