Recombinant human ICT1 protein
Peptidyl-tRNA hydrolase ICT1 acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. It may be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes. Recombinant human ICT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Peptidyl-tRNA hydrolase ICT1 mitochondrial, Peptidyl-tRNA hydrolase ICT1, mitochondrial, DS-1, DS1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
22.8 kDa (200aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Richter R., et al. (2010) EMBO J. 29:1116-1125; ; van Belzen N., et al. (1995) Eur. J. Biochem. 234:843-848;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 30% glycerol, 1mM DTT
Scientific Category
Cancer
NCBI Accession Number
NP_001536
Uniprot Accession Number
Q14197
Species
Human
AA Sequence
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