Recombinant human RNA polymerase II subunit RPB9/POLR2I protein

POLR2I, also known as RPB9, belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family. This protein is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Also it is synthesizes mRNA precursors and many functional non-coding RNAs. Recombinant human PORL2I protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

DNA-directed RNA polymerase II subunit RPB9, hRPB14.5, RPB9

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

17 kDa (148aa) confirmed by MALDI-TOF

Additionnal Information

POLR2I, DNA-directed RNA polymerase II subunit RPB9, hRPB14.5, RPB9, ATGP1828-10 µg, ATGP1828-20 µg, ATGP1828-50 µg, ATGP1828-100 µg, ATGP1828-250 µg, ATGP1828-500 µg, ATGP1828-1 mg, ATGP1828-10, ATGP1828-20, ATGP1828-50, ATGP1828-100, ATGP1828-250, ATGP1828-500, ATGP1828-1

References & Citations

Kershnar E., et al. (1998) J. Biol. Chem. 273:34444-34453

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_006224

Uniprot Accession Number

P36954

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMEPDGTY EPGFVGIRFC QECNNMLYPK EDKENRILLY ACRNCDYQQE ADNSCIYVNK ITHEVDELTQ IIADVSQDPT LPRTEDHPCQ KCGHKEAVFF QSHSARAEDA MRLYYVCTAP HCGHRWTE