Recombinant human MRRF protein

MRRF binds to the large ribosomal subunit in the cleft that contains the peptidyl transferase center. This protein is responsible for the release of ribosome from messenger RNA at the termination of protein biosynthesis. Also, it may increase the efficiency of translation by recycling ribosome from one round of translation to another. Recombinant human MRRF protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ribosome-recycling factor mitochondrial isoform 1, Ribosome-recycling factor, mitochondrial isoform 1, MRFF, MTRRF, RRF

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

25.1 kDa (228aa) confirmed by MALDI-TOF

Additionnal Information

MRRF, Ribosome-recycling factor mitochondrial isoform 1, Ribosome-recycling factor, mitochondrial isoform 1, MRFF, MTRRF, RRF, ATGP1826-10 µg, ATGP1826-20 µg, ATGP1826-50 µg, ATGP1826-100 µg, ATGP1826-250 µg, ATGP1826-500 µg, ATGP1826-1 mg, ATGP1826-10, ATGP1826-20, ATGP1826-50, ATGP1826-100, ATGP1826-250, ATGP1826-500, ATGP1826-1

References & Citations

Hirokawa G., et al. (2008) Nucleic Acids Res. 36 (21) :6676-87; ; Pai RD., et al. (2008 J Mol Biol. 376 (5) :1334-47.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 30% glycerol, 2mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_620132

Uniprot Accession Number

Q96E11

Species

Human

AA Sequence

ATKKAKAKG KGQSQTRVNI NAALVEDIIN LEEVNEEMKS VIEALKDNFN KTLNIRTSPG SLDKIAVVTA DGKLALNQIS QISMKSPQLI LVNMASFPEC TAAAIKAIRE SGMNLNPEVE GTLIRVPIPQ VTREHREMLV KLAKQNTNKA KDSLRKVRTN SMNKLKKSKD TVSEDTIRLI EKQISQMADD TVAELDRHLA VKTKELLG