Recombinant human MRRF protein
MRRF binds to the large ribosomal subunit in the cleft that contains the peptidyl transferase center. This protein is responsible for the release of ribosome from messenger RNA at the termination of protein biosynthesis. Also, it may increase the efficiency of translation by recycling ribosome from one round of translation to another. Recombinant human MRRF protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Ribosome-recycling factor mitochondrial isoform 1, Ribosome-recycling factor, mitochondrial isoform 1, MRFF, MTRRF, RRF
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
25.1 kDa (228aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Hirokawa G., et al. (2008) Nucleic Acids Res. 36 (21) :6676-87; ; Pai RD., et al. (2008 J Mol Biol. 376 (5) :1334-47.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 30% glycerol, 2mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_620132
Uniprot Accession Number
Q96E11
Species
Human
AA Sequence
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