Recombinant human HAI-2A protein

SPINT2 is a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. This protein is a putative tumor suppressor, and mutations in this protein result in congenital sodium diarrhea. Recombinant human SPINT2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Kunitz-type protease inhibitor 2, DIAR3, FLJ45571, HAI-2, HAI2, Kop, PB

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

21.8 kDa (195aa)

Additionnal Information

HAI2A, Kunitz-type protease inhibitor 2, DIAR3, FLJ45571, HAI-2, HAI2, Kop, PB, ATGP1823-10 µg, ATGP1823-20 µg, ATGP1823-50 µg, ATGP1823-100 µg, ATGP1823-250 µg, ATGP1823-500 µg, ATGP1823-1 mg, ATGP1823-10, ATGP1823-20, ATGP1823-50, ATGP1823-100, ATGP1823-250, ATGP1823-500, ATGP1823-1

References & Citations

Bergum, C.et al. (2010) Prostate 70 (13), 1422-1428 ; ; Huang, H.P., et al. (2012) Hepatology 55 (1), 161-172;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea 10% glycerol

Scientific Category

Development

NCBI Accession Number

NP_066925

Uniprot Accession Number

O43291

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMADRER SIHDFCLVSK VVGRCRASMP RWWYNVTDGS CQLFVYGGCD GNSNNYLTKE ECLKKCATVT ENATGDLATS RNAADSSVPS APRRQDSEDH SSDMFNYEEY CTANAVTGPC RASFPRWYFD VERNSCNNFI YGGCRGNKNS YRSEEACMLR CFRQQENPPL PLGSK