Recombinant human NKG2D/KLRK1 protein

KLRK1 is an activating receptor that has recently generated considerable interest. The most intriguing of these are a pair of closely related proteins called MICA and MICB. These are cell-surface molecules distantly related to MHC class I proteins, and the genes possess elements of heat shock promoters. MICA and MICB, therefore, are expressed during cell stress and are up-regulated in tumor cells and during viral infections. This receptor-ligand combination may play a critical role in the immune response to a variety of pathologies. Recombinant human KLRK1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Killer cell lectin like receptor K1, Killer cell lectin-like receptor subfamily K member 1, NKG2-D type II integral membrane protein, NK cell receptor D, NKG2-D-activating NK receptor, CD314, NKG2D, KLR, NKG2-D

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

19.2 kDa (168aa)

Additionnal Information

NKG2D, NKG2-D type II integral membrane protein, CD314, D12S2489E, KLR, NKG2-D, NKG2D, ATGP1818-10 µg, ATGP1818-20 µg, ATGP1818-50 µg, ATGP1818-100 µg, ATGP1818-250 µg, ATGP1818-500 µg, ATGP1818-1 mg, ATGP1818-10, ATGP1818-20, ATGP1818-50, ATGP1818-100, ATGP1818-250, ATGP1818-500, ATGP1818-1

References & Citations

Guerra N. et al. (2008) Immunity. 28:571-580.; ; Ravetch JV. et al. (2000) Science. 290:84-89.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea

Scientific Category

Immunology

NCBI Accession Number

NP_031386

Uniprot Accession Number

P26718

Species

Human

AA Sequence

IWSAVF LNSLFNQEVQ IPLTESYCGP CPKNWICYKN NCYQFFDESK NWYESQASCM SQNASLLKVY SKEDQDLLKL VKSYHWMGLV HIPTNGSWQW EDGSILSPNL LTIIEMQKGD CALYASSFKG YIENCSTPNT YICMQRTV