Recombinant human AMPK beta 2 protein

PRKAB2 is a regulatory subunit of the AMP-activated protein kinase (AMPK) . AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. PRKAB2 may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles. Recombinant human PRKAB2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

5'-AMP-activated protein kinase subunit beta-2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

32.8 kDa (296aa)

Additionnal Information

AMPK b2, 5'-AMP-activated protein kinase subunit beta-2, ATGP1817-10 µg, ATGP1817-20 µg, ATGP1817-50 µg, ATGP1817-100 µg, ATGP1817-250 µg, ATGP1817-500 µg, ATGP1817-1 mg, ATGP1817-10, ATGP1817-20, ATGP1817-50, ATGP1817-100, ATGP1817-250, ATGP1817-500, ATGP1817-1

References & Citations

Souza RP. et al. (2012) J Psychiatr Res. 46:462-468.; ; Jung KC. et al. (2003) Cytogenet Genome Res. 103:202C.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 2M urea

Scientific Category

Metabolism

NCBI Accession Number

NP_005390

Uniprot Accession Number

O43741

Species

Human

AA Sequence

MGNTTS DRVSGERHGA KAARSEGAGG HAPGKEHKIM VGSTDDPSVF SLPDSKLPGD KEFVSWQQDL EDSVKPTQQA RPTVIRWSEG GKEVFISGSF NNWSTKIPLI KSHNDFVAIL DLPEGEHQYK FFVDGQWVHD PSEPVVTSQL GTINNLIHVK KSDFEVFDAL KLDSMESSET SCRDLSSSPP GPYGQEMYAF RSEERFKSPP ILPPHLLQVI LNKDTNISCD PALLPEPNHV MLNHLYALSI KDSVMVLSAT HRYKKKYVTT LLYKPI