Recombinant human RASSF1A protein

RASSF1A is similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. This protein required for death receptor-dependent apoptosis. Recombinant human RASSF1A protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Ras association domain-containing protein 1, 123F2, NORE2A, RASSF1, RDA32, REH3P21

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

42.9 kDa (376aa)

Additionnal Information

RASSF1A, Ras association domain-containing protein 1, 123F2, NORE2A, RASSF1, RDA32, REH3P21, ATGP1815-10 µg, ATGP1815-20 µg, ATGP1815-50 µg, ATGP1815-100 µg, ATGP1815-250 µg, ATGP1815-500 µg, ATGP1815-1 mg, ATGP1815-10, ATGP1815-20, ATGP1815-50, ATGP1815-100, ATGP1815-250, ATGP1815-500, ATGP1815-1

References & Citations

Burbee D.G., et al. (2001) J. Natl. Cancer Inst. 93:691-699; ; Shivakumar L., et al. (2002) Mol. Cell. Biol. 22:4309-4318;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_009113

Uniprot Accession Number

Q9NS23

Species

Human

AA Sequence

MSGE PELIELRELA PAGRAGKGRT RLERANALRI ARGTACNPTR QLVPGRGHRF QPAGPATHTW CDLCGDFIWG VVRKGLQCAH CKFTCHYRCR ALVCLDCCGP RDLGWEPAVE RDTNVDEPVE WETPDLSQAE IEQKIKEYNA QINSNLFMSL NKDGSYTGFI KVQLKLVRPV SVPSSKKPPS LQDARRGPGR GTSVRRRTSF YLPKDAVKHL HVLSRTRARE VIEALLRKFL VVDDPRKFAL FERAERHGQV YLRKLLDDEQ PLRLRLLAGP SDKALSFVLK ENDSGEVNWD AFSMPELHNF LRILQREEEE HLRQILQKYS YCRQKIQEAL HACPLG