Recombinant human Endophilin B2/SH3GLB2 protein

Endophilin-B2, also known as SH3GLB2, is a member of the endophilin B subgroup. The endophilins comprise a family of proteins that associate with amphiphysin, synaptojanin and dynamin and are implicated in presynaptic vesicle trafficking at nerve terminals. The expression patterns of the endophilins are consistent with their cellular functions at the neuronal synapse. SH3GLB2 is ubiquitously expressed but shows highest levels in brain, adult lung, ovary, and spinal cord. A decreased level of SH3GLB2 is found in Down syndrome and may reflect brain dysgenesis. Recombinant human SH3GLB2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Endophilin-B2, PP6569, PP9455

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

46.4 kDa (418aa) confirmed by MALDI-TOF

Additionnal Information

SH3GLB2, PP9455, PP6569, EndophilinB2, Endophilin B2, Endophilin-B2, ATGP1814-10 µg, ATGP1814-20 µg, ATGP1814-50 µg, ATGP1814-100 µg, ATGP1814-250 µg, ATGP1814-500 µg, ATGP1814-1 mg, ATGP1814-010, ATGP1814-020, ATGP1814-050, ATGP1814-100, ATGP1814-250, ATGP1814-500, ATGP1814-01M

References & Citations

Pierrat B., et al. (2001) Genomics. 71:222-234; ; Engidawork E., et al. (2003) Neuroscience. 122: 145-154.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol,1mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_064530

Uniprot Accession Number

Q9NR46

Species

Human

AA Sequence

MDFNMKK LASDAGIFFT RAVQFTEEKF GQAEKTELDA HFENLLARAD STKNWTEKIL RQTEVLLQPN PSARVEEFLY EKLDRKVPSR VTNGELLAQY MADAASELGP TTPYGKTLIK VAEAEKQLGA AERDFIHTAS ISFLTPLRNF LEGDWKTISK ERRLLQNRRL DLDACKARLK KAKAAEAKAT TVPDFQETRP RNYILSASAS ALWNDEVDKA EQELRVAQTE FDRQAEVTRL LLEGISSTHV NHLRCLHEFV KSQTTYYAQC YRHMLDLQKQ LGRFPGTFVG TTEPASPPLS STSPTTAAAT MPVVPSVASL APPGEASLCL EEVAPPASGT RKARVLYDYE AADSSELALL ADELITVYSL PGMDPDWLIG ERGNKKGKVP VTYLELLS