Recombinant human Troponin I2/TNNI2 protein

Troponin I, fast skeletal muscle, also known as TNNI2, is a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible for the calcium-dependent regulation of striated muscle contraction. This protein also suppresses tumor growth in human ovarian carcinoma. Mutations in TNNI2 cause myopathy and distal arthrogryposis type 2B. Recombinant human TNNI2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Troponin I fast skeletal muscle, AMCD2B, DA2B, FSSV, fsTnI

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

23.5 kDa (202aa)

Additionnal Information

TNNI2, Troponin I fast skeletal muscle, Troponin I, fast skeletal muscle, AMCD2B, DA2B, FSSV, fsTnI, ATGP1810-10 µg, ATGP1810-20 µg, ATGP1810-50 µg, ATGP1810-100 µg, ATGP1810-250 µg, ATGP1810-500 µg, ATGP1810-1 mg, ATGP1810-10, ATGP1810-20, ATGP1810-50, ATGP1810-100, ATGP1810-250, ATGP1810-500, ATGP1810-1

References & Citations

Ausoni S., et al. (1994) J Biol Chem. 269:339-346.; ; Potter J D., et al. (1995) J Biol Chem. 270:2557-2562.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2M urea, 20% glycerol, 0.2M NaCl

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_001139301

Uniprot Accession Number

P48788

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGDEEKRNRA ITARRQHLKS VMLQIAATEL EKEESRREAE KQNYLAEHCP PLHIPGSMSE VQELCKQLHA KIDAAEEEKY DMEVRVQKTS KELEDMNQKL FDLRGKFKRP PLRRVRMSAD AMLKALLGSK HKVCMDLRAN LKQVKKEDTE KERDLRDVGD WRKNIEEKSG MEGRKKMFES ES