Recombinant human RNASE3 protein

RNASE3 is cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. It possesses a wide variety of biological activities. This protein exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains and promotes E. coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content. Recombinant human RNASE3 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Eosinophil cationic protein, ECP; RNS3, Ribonuclease 3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

19.9 kDa (171aa)

Additionnal Information

RNASE3, Eosinophil cationic protein, ECP; RNS3, Ribonuclease 3, ATGP1809-10 µg, ATGP1809-20 µg, ATGP1809-50 µg, ATGP1809-100 µg, ATGP1809-250 µg, ATGP1809-500 µg, ATGP1809-1 mg, ATGP1809-10, ATGP1809-20, ATGP1809-50, ATGP1809-100, ATGP1809-250, ATGP1809-500, ATGP1809-1

References & Citations

Adu B, Dodoo D, Adukpo S, et al. (2011) . PLoS One. 6 (12) :e29465.; ; Torrent M, Nogues MV, Boix E, et al. (2011) . J Mol Recognit. 24 (1) :90-100.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

AAH96060

Uniprot Accession Number

P12724

Species

Human

AA Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMRP PQFTRAQWFA IQHISLNPPR CTIAMRAINN YRWRCKNQNT FLRTTFANVV NVCGNQSIRC PHNRTLNNCH RSRFRVPLLH CDLINPGAQN ISNCRYADRP GRRFYVVACD NRDPRDSPRY PVVPVHLDTT I