Recombinant human MAPKAPK3 protein
MAP kinase-activated protein kinase 3, also known as MAPKAPK3, is involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating Au-rich elements (AREs) -binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Recombinant human MAPKAPK3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
MAP kinase-activated protein kinase 3, 3PK, MAPKAP-K3, MAPKAP3, MAPKAPK-3, MK-3
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
45.4 kDa (405aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Voncken J.W., et al. (2005) J. Biol. Chem. 280:5178-5187; ; Mendoza H., et al. (2008) Biochem. J. 409:711-722;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 1mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
NP_004626
Uniprot Accession Number
Q16644
Species
Human
AA Sequence
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