Recombinant human MAPKAPK3 protein

MAP kinase-activated protein kinase 3, also known as MAPKAPK3, is involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating Au-rich elements (AREs) -binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Recombinant human MAPKAPK3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

MAP kinase-activated protein kinase 3, 3PK, MAPKAP-K3, MAPKAP3, MAPKAPK-3, MK-3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

45.4 kDa (405aa) confirmed by MALDI-TOF

Additionnal Information

MAPKAPK3, MAP kinase-activated protein kinase 3, 3PK, MAPKAP-K3, MAPKAP3, MAPKAPK-3, MK-3, ATGP1805-10 µg, ATGP1805-20 µg, ATGP1805-50 µg, ATGP1805-100 µg, ATGP1805-250 µg, ATGP1805-500 µg, ATGP1805-1 mg, ATGP1805-10, ATGP1805-20, ATGP1805-50, ATGP1805-100, ATGP1805-250, ATGP1805-500, ATGP1805-1

References & Citations

Voncken J.W., et al. (2005) J. Biol. Chem. 280:5178-5187; ; Mendoza H., et al. (2008) Biochem. J. 409:711-722;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_004626

Uniprot Accession Number

Q16644

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMDGETAE EQGGPVPPPV APGGPGLGGA PGGRREPKKY AVTDDYQLSK QVLGLGVNGK VLECFHRRTG QKCALKLLYD SPKARQEVDH HWQASGGPHI VCILDVYENM HHGKRCLLII MECMEGGELF SRIQERGDQA FTEREAAEIM RDIGTAIQFL HSHNIAHRDV KPENLLYTSK EKDAVLKLTD FGFAKETTQN ALQTPCYTPY YVAPEVLGPE KYDKSCDMWS LGVIMYILLC GFPPFYSNTG QAISPGMKRR IRLGQYGFPN PEWSEVSEDA KQLIRLLLKT DPTERLTITQ FMNHPWINQS MVVPQTPLHT ARVLQEDKDH WDEVKEEMTS ALATMRVDYD QVKIKDLKTS NNRLLNKRRK KQAGSSSASQ GCNNQ