Recombinant human PSMD9 protein

PSMD9 acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC) . During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process. Recombinant human PSMD9 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

26S proteasome non-ATPase regulatory subunit 9, p27, Rpn4

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

27.1 kDa (246aa) confirmed by MALDI-TOF

Additionnal Information

PSMD9, 26S proteasome non-ATPase regulatory subunit 9, p27, Rpn4, ATGP1804-10 µg, ATGP1804-20 µg, ATGP1804-50 µg, ATGP1804-100 µg, ATGP1804-250 µg, ATGP1804-500 µg, ATGP1804-1 mg, ATGP1804-10, ATGP1804-20, ATGP1804-50, ATGP1804-100, ATGP1804-250, ATGP1804-500, ATGP1804-1

References & Citations

Kaneko T., et al. (2009) Cell. 137:914-925; ; Watanabe T.K., et al. (1998) Genomics. 50:241-250;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Ubiquitination

NCBI Accession Number

NP_002804

Uniprot Accession Number

O00233

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSDEEAR QSGGSSQAGV VTVSDVQELM RRKEEIEAQI KANYDVLESQ KGIGMNEPLV DCEGYPRSDV DLYQVRTARH NIICLQNDHK AVMKQVEEAL HQLHARDKEK QARDMAEAHK EAMSRKLGQS ESQGPPRAFA KVNSISPGSP ASIAGLQVDD EIVEFGSVNT QNFQSLHNIG SVVQHSEGKP LNVTVIRRGE KHQLRLVPTR WAGKGLLGCN IIPLQR