Recombinant human AP3S1 protein

AP-3 complex subunit sigma-1, also known as AP3S1, is a Part of the AP-3 complex, an adapter-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. Recombinant human AP3S1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

AP-3 complex subunit sigma-1, CLAPS3, Sigma3A

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

23.8 kDa (213aa) confirmed by MALDI-TOF

Additionnal Information

AP3S1, AP-3 complex subunit sigma-1, CLAPS3, Sigma3A, ATGP1803-10 µg, ATGP1803-20 µg, ATGP1803-50 µg, ATGP1803-100 µg, ATGP1803-250 µg, ATGP1803-500 µg, ATGP1803-1 mg, ATGP1803-10, ATGP1803-20, ATGP1803-50, ATGP1803-100, ATGP1803-250, ATGP1803-500, ATGP1803-1

References & Citations

Burkard T.R., et al. (2011) BMC Syst. Biol. 5:17-17; ; Dell'Angelica E.C., et al. (1997) EMBO J. 16:917-928;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Transportation

NCBI Accession Number

NP_001275

Uniprot Accession Number

Q92572

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MIKAILIFNN HGKPRLSKFY QPYSEDTQQQ IIRETFHLVS KRDENVCNFL EGGLLIGGSD NKLIYRHYAT LYFVFCVDSS ESELGILDLI QVFVETLDKC FENVCELDLI FHVDKVHNIL AEMVMGGMVL ETNMNEIVTQ IDAQNKLEKS EAGLAGAPAR AVSAVKNMNL PEIPRNINIG DISIKVPNLP SFK