Recombinant human MRPL28 protein

MRPL28, also as known as 39S ribosomal protein L28, mitochondrial, is a mitochondrial protein that belongs to the ribosomal protein L28P family. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPL28 potentially represents an important therapeutic reagent for HLA-A24 (A24) patients as this antigen is recognized by tumor-infiltrating lymphocyte (TIL) 1290, which targets the A24 serotype. This protein is found in a variety of normal tissues including spleen, testis, thymus, liver, kidney, brain, adrenal, lung and retinal tissue. Recombinant human MRPL28 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

39S ribosomal protein L28 mitochondrial, 39S ribosomal protein L28, mitochondrial, MAAT1, p15

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

25.8 kDa (222aa) confirmed by MALDI-TOF

Additionnal Information

MRPL28, 39S ribosomal protein L28 mitochondrial, 39S ribosomal protein L28, mitochondrial, MAAT1, p15, ATGP1802-10 µg, ATGP1802-20 µg, ATGP1802-50 µg, ATGP1802-100 µg, ATGP1802-250 µg, ATGP1802-500 µg, ATGP1802-1 mg, ATGP1802-10, ATGP1802-20, ATGP1802-50, ATGP1802-100, ATGP1802-250, ATGP1802-500, ATGP1802-1

References & Citations

Koc EC, et al. (2001) J Biol Chem 276 (47) : 43958-69.; ; Kawakami, Y., et al. (2000) J.Immunother. 23: 17-27.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_006419

Uniprot Accession Number

Q13084

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MNGQRERVED VPIPIYFPPE SQRGLWGGEG WILGQIYANN DKLSKRLKKV WKPQLFEREF YSEILDKKFT VTVTMRTLDL IDEAYGLDFY ILKTPKEDLC SKFGMDLKRG MLLRLARQDP QLHPEDPERR AAIYDKYKEF AIPEEEAEWV GLTLEEAIEK QRLLEEKDPV PLFKIYVAEL IQQLQQQALS EPAVVQKRAS GQ