Recombinant human Endophilin B1/Bif-1 protein
SH3GLB1, also as known as endophilin-B1, belongs to the endophilin family. Endophilin B1 is a member of the B subgroup of the endophilin family that is required for maintenance of mitochondrial morphology and for the regulation of the outer mitochondrial membrane dynamics. This protein required for normal outer mitochondrial membrane dynamics. Also it required for coatomer-mediated retrograde transport in certain cells. SH3GLB1 has been shown to interact with SH3GLB2 and Bcl-2-associated X protein. This protein highly expressed in heart, skeletal muscle, kidney and placenta. Recombinant human SH3GLB1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Endophilin-B1, Bif-1, CGI-61, dJ612B15.2, PPP1R70
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
41.9 kDa (373aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Pierrat B., et al. (2001) Genomics 71:222-234; ; Modregger J., et al. (2003) J. Biol. Chem. 278:4160-4167;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 2mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_057093.1
Uniprot Accession Number
Q9Y371
Species
Human
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