Recombinant human ACAT1 protein

ACAT1, also known as acetoacetyl-CoA thiolase, is an enzyme belongs to belongs to the membrane-bound acyltransferase family and Sterol o-acyltransferase subfamily. ACAT1 is an acetyl-CoA C-acetyltransferase enzyme. This enzyme catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. It plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase. Recombinant human ACAT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Acetyl-CoA acetyltransferase 1, ACAT, MAT, T2, THIL

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

43.8 kDa (417aa) confirmed by MALDI-TOF

Additionnal Information

ACAT1, acetyl-CoA acetyltransferase 1, ACAT, MAT, T2, THIL, ATGP1800-10 µg, ATGP1800-20 µg, ATGP1800-50 µg, ATGP1800-100 µg, ATGP1800-250 µg, ATGP1800-500 µg, ATGP1800-1 mg, ATGP1800-10, ATGP1800-20, ATGP1800-50, ATGP1800-100, ATGP1800-250, ATGP1800-500, ATGP1800-1

References & Citations

Haapalainen, A.M., et al. (2007) Biochemistry 46: 4305-4321.; ; Thompson, S.L. et al. (1990) J. Biol. Chem. 265:5731-5735.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_000010

Uniprot Accession Number

P24752

Species

Human

AA Sequence

VSKPTLK EVVIVSATRT PIGSFLGSLS LLPATKLGSI AIQGAIEKAG IPKEEVKEAY MGNVLQGGEG QAPTRQAVLG AGLPISTPCT TINKVCASGM KAIMMASQSL MCGHQDVMVA GGMESMSNVP YVMNRGSTPY GGVKLEDLIV KDGLTDVYNK IHMGSCAENT AKKLNIARNE QDAYAINSYT RSKAAWEAGK FGNEVIPVTV TVKGQPDVVV KEDEEYKRVD FSKVPKLKTV FQKENGTVTA ANASTLNDGA AALVLMTADA AKRLNVTPLA RIVAFADAAV EPIDFPIAPV YAASMVLKDV GLKKEDIAMW EVNEAFSLVV LANIKMLEID PQKVNINGGA VSLGHPIGMS GARIVGHLTH ALKQGEYGLA SICNGGGGAS AMLIQKL