Recombinant human ACAT1 protein
ACAT1, also known as acetoacetyl-CoA thiolase, is an enzyme belongs to belongs to the membrane-bound acyltransferase family and Sterol o-acyltransferase subfamily. ACAT1 is an acetyl-CoA C-acetyltransferase enzyme. This enzyme catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. It plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase. Recombinant human ACAT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Acetyl-CoA acetyltransferase 1, ACAT, MAT, T2, THIL
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
43.8 kDa (417aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Haapalainen, A.M., et al. (2007) Biochemistry 46: 4305-4321.; ; Thompson, S.L. et al. (1990) J. Biol. Chem. 265:5731-5735.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 0.1M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_000010
Uniprot Accession Number
P24752
Species
Human
AA Sequence
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