Recombinant human SLD5/GINS4 protein

GINS4 plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS4 is important for GINS complex assembly. GINS complex seems to bind preferentially to single-stranded DNA. Recombinant human GINS4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

DNA replication complex GINS protein SLD5, GINS complex subunit 4, GINS4, SLD5

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

28.2 kDa (243aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

GINS4, DNA replication complex GINS protein SLD5, SLD5, GINS complex subunit 4, ATGP1792-10 µg, ATGP1792-20 µg, ATGP1792-50 µg, ATGP1792-100 µg, ATGP1792-250 µg, ATGP1792-500 µg, ATGP1792-1 mg, ATGP1792-10, ATGP1792-20, ATGP1792-50, ATGP1792-100, ATGP1792-250, ATGP1792-500, ATGP1792-1

References & Citations

Kamada K., et al. (2007) Nat. Struct. Mol. Biol. 14:388-396; ; Chang Y.P., et al. (2007) Proc. Natl. Acad. Sci. u.S.A. 104:12685-12690;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_115712

Uniprot Accession Number

Q9BRT9

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MTEEVDFLGQ DSDGGSEEVV LTPAELIERL EQAWMNEKFA PELLESKPEI VECVMEQLEH MEENLRRAKR EDLKVSIHQM EMERIRYVLS SYLRCRLMKI EKFFPHVLEK EKTRPEGEPS SLSPEELAFA REFMANTESY LKNVALKHMP PNLQKVDLFR AVPKPDLDSY VFLRVRERQE NILVEPDTDE QRDYVIDLEK GSQHLIRYKT IAPLVASGAV QLI