Recombinant human ASMT protein

ASMT belongs to the methyltransferase superfamily and is involved in melatonin biosynthesis. Expressed in brain, retina and pineal gland, ASMT functions to catalyze the final reaction in the synthesis of melatonin, specifically the conversion of S-adenosyl-L-methionine and N-acetylserotonin to S-adenosyl-L-homocysteine and melatonin. Recombinant human ASMT protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Acetylserotonin O-methyltransferase, HIOMT, HIOMTY

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

35.3 kDa (318aa)

Additionnal Information

ASMT, Acetylserotonin O-methyltransferase, HIOMT, HIOMTY, ATGP1789-10 µg, ATGP1789-20 µg, ATGP1789-50 µg, ATGP1789-100 µg, ATGP1789-250 µg, ATGP1789-500 µg, ATGP1789-1 mg, ATGP1789-10, ATGP1789-20, ATGP1789-50, ATGP1789-100, ATGP1789-250, ATGP1789-500, ATGP1789-1

References & Citations

Rodriguez I R., et al. (1994) J Biol Chem. 269:31969-31977.; ; Toma C., et al. (2007) Mol Psychiatry. 12:977-979.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1M urea, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

AAH01620

Uniprot Accession Number

P46597

Species

Human

AA Sequence

MGSSEDQAYR LLNDYANGFM VSQVLFAACE LGVFDLLAEA PGPLDVAAVA AGVRASAHGT ELLLDICVSL KLLKVETRGG KAFYRNTELS SDYLTTVSPT SQCSMLKYMG RTSYRCWGHL ADAVREGRNQ YLETFGVPAE ELFTAIYRSE GERLQFMQAL QEVWSVNGRS VLTAFDLSVF PLMCDLGGDF FKDPLPEADL YILARVLHDW ADGKCSHLLE RIYHTCKPGG GILVIESLLD EDRRGPLLTQ LYSLNMLVQT EGQERTPTHY HMLLSSAGFR DFQFKKTGAI YDAILARK