Recombinant human UMPS protein

Uridine 5'-monophosphate synthase, also known as UMPS, is a bifunctional enzyme that catalyzes the final two steps of the de novo pyrimidine biosynthetic pathway. unlike prokaryotes, UMPS in eukaryotes combines the orotate phosphoribosyltransferase and the orotidine-5'-monophosphate (OMP) decarboxylase activities into a single protein. The union of these two enzymes is thought to stabilize the catalytic centers due to the low molar concentration of the protein in mammalian cells. Defects in this gene are the cause of hereditary orotic aciduria. Recombinant human UMPS protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Uridine 5'-monophosphate synthase, OPRT

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

54.3 kDa (500aa)

Additionnal Information

UMPS, uridine 5'-monophosphate synthase, OPRT, ATGP1788-10 µg, ATGP1788-20 µg, ATGP1788-50 µg, ATGP1788-100 µg, ATGP1788-250 µg, ATGP1788-500 µg, ATGP1788-1 mg, ATGP1788-10, ATGP1788-20, ATGP1788-50, ATGP1788-100, ATGP1788-250, ATGP1788-500, ATGP1788-1

References & Citations

Suchi M., et al. (1997) Genet. 60:525-539.; ; Yablonski M J., et al. (1996) J Biol Chem. 271:10704-10708.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2M urea, 20% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_000364

Uniprot Accession Number

P11172

Species

Human

AA Sequence

MAVARAALGP LVTGLYDVQA FKFGDFVLKS GLSSPIYIDL RGIVSRPRLL SQVADILFQT AQNAGISFDT VCGVPYTALP LATVICSTNQ IPMLIRRKET KDYGTKRLVE GTINPGETCL IIEDVVTSGS SVLETVEVLQ KEGLKVTDAI VLLDREQGGK DKLQAHGIRL HSVCTLSKML EILEQQKKVD AETVGRVKRF IQENVFVAAN HNGSPLSIKE APKELSFGAR AELPRIHPVA SKLLRLMQKK ETNLCLSADV SLARELLQLA DALGPSICML KTHVDILNDF TLDVMKELIT LAKCHEFLIF EDRKFADIGN TVKKQYEGGI FKIASWADLV NAHVVPGSGV VKGLQEVGLP LHRGCLLIAE MSSTGSLATG DYTRAAVRMA EEHSEFVVGF ISGSRVSMKP EFLHLTPGVQ LEAGGDNLGQ QYNSPQEVIG KRGSDIIIVG RGIISAADRL EAAEMYRKAA WEAYLSRLGV