Recombinant human NEK7 protein
NEK7 is protein kinase which plays an important role in mitotic cell cycle progression. It is required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. NEK7 is highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Recombinant human NEK7 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Serine/threonine-protein kinase Nek7, NIMA (never in mitosis gene a) -related kinase 7
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
37 kDa (326aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Yissachar N, Salem H, et al. (2006) . FEBS Lett. 580 (27) :6489-95. ; ; Kim S, Lee K, et al. (2007) . Biochem Biophys Res Commun. 360 (1) :56-62. ;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol
Scientific Category
Cell Cycle
NCBI Accession Number
NP_598001
Uniprot Accession Number
Q8TDX7
Species
Human
AA Sequence
Frequently Asked Questions
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