Recombinant human NEK7 protein

NEK7 is protein kinase which plays an important role in mitotic cell cycle progression. It is required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. NEK7 is highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Recombinant human NEK7 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Serine/threonine-protein kinase Nek7, NIMA (never in mitosis gene a) -related kinase 7

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

37 kDa (326aa) confirmed by MALDI-TOF

Additionnal Information

NEK7, Serine/threonine-protein kinase Nek7, NIMA (never in mitosis gene a) -related kinase 7, ATGP1787-10 µg, ATGP1787-20 µg, ATGP1787-50 µg, ATGP1787-100 µg, ATGP1787-250 µg, ATGP1787-500 µg, ATGP1787-1 mg, ATGP1787-10, ATGP1787-20, ATGP1787-50, ATGP1787-100, ATGP1787-250, ATGP1787-500, ATGP1787-1

References & Citations

Yissachar N, Salem H, et al. (2006) . FEBS Lett. 580 (27) :6489-95. ; ; Kim S, Lee K, et al. (2007) . Biochem Biophys Res Commun. 360 (1) :56-62. ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_598001

Uniprot Accession Number

Q8TDX7

Species

Human

AA Sequence

MDEQSQ GMQGPPVPQF QPQKALRPDM GYNTLANFRI EKKIGRGQFS EVYRAACLLD GVPVALKKVQ IFDLMDAKAR ADCIKEIDLL KQLNHPNVIK YYASFIEDNE LNIVLELADA GDLSRMIKHF KKQKRLIPER TVWKYFVQLC SALEHMHSRR VMHRDIKPAN VFITATGVVK LGDLGLGRFF SSKTTAAHSL VGTPYYMSPE RIHENGYNFK SDIWSLGCLL YEMAALQSPF YGDKMNLYSL CKKIEQCDYP PLPSDHYSEE LRQLVNMCIN PDPEKRPDVT YVYDVAKRMH ACTASS