Recombinant human AMPK beta 1/PRKAB1 protein

5'-AMP-activated protein kinase subunit beta-1, also known as PRKAB1, inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3) . Recombinant human PRKAB1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

5'-AMP-activated protein kinase subunit beta-1, AMPK, HAMPKb, PRKAB1, Protein kinase AMP-activated non-catalytic subunit beta 1, AMPK subunit beta-1, AMPKb

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

32.8 kDa (293aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

AMPK b1, 5'-AMP-activated protein kinase subunit beta-1, AMPK, HAMPKb, ATGP1786-10 µg, ATGP1786-20 µg, ATGP1786-50 µg, ATGP1786-100 µg, ATGP1786-250 µg, ATGP1786-500 µg, ATGP1786-1 mg, ATGP1786-10, ATGP1786-20, ATGP1786-50, ATGP1786-100, ATGP1786-250, ATGP1786-500, ATGP1786-1

References & Citations

Hardie D.G., et al. (2007) Nat. Rev. Mol. Cell Biol. 8:774-785; ; Towler M.C., et al. (2007) Circ. Res. 100:328-341;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_006244.2

Uniprot Accession Number

Q9Y478

Species

Human

AA Sequence

MGNTSSE RAALERHGGH KTPRRDSSGG TKDGDRPKIL MDSPEDADLF HSEEIKAPEK EEFLAWQHDL EVNDKAPAQA RPTVFRWTGG GKEVYLSGSF NNWSKLPLTR SHNNFVAILD LPEGEHQYKF FVDGQWTHDP SEPIVTSQLG TVNNIIQVKK TDFEVFDALM VDSQKCSDVS ELSSSPPGPY HQEPYVCKPE ERFRAPPILP PHLLQVILNK DTGISCDPAL LPEPNHVMLN HLYALSIKDG VMVLSATHRY KKKYVTTLLY KPI