Recombinant human NDUFS3 protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, also known as NDuFS3, belongs to the complex I 30 kDa subunit family. The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. The iron-sulfur protein (IP) fraction of complex I is made up of 7 subunits. Recombinant human NDuFS3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, CI-30, NADH:ubiquinone oxidoreductase core subunit S3, Complex I-30kD, CI-30kD, NADH-ubiquinone oxidoreductase 30 kDa subunit, NADH dehydrogenase (ubiquinone) Fe-S protein 3 30kDa, NADH-coenzyme Q reductase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

28.7 kDa (249aa) confirmed by MALDI-TOF

Additionnal Information

NDUFS3, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, CI-30, ATGP1785-10 µg, ATGP1785-20 µg, ATGP1785-50 µg, ATGP1785-100 µg, ATGP1785-250 µg, ATGP1785-500 µg, ATGP1785-1 mg, ATGP1785-10, ATGP1785-20, ATGP1785-50, ATGP1785-100, ATGP1785-250, ATGP1785-500, ATGP1785-1

References & Citations

Murray J., et al. (2003) J. Biol. Chem. 278:13619-13622; ; Saada A., et al. (2009) Am. J. Hum. Genet. 84:718-727;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_004542

Uniprot Accession Number

O75489

Species

Human

AA Sequence

ESAGADTRP TVRPRNDVAH KQLSAFGEYV AEILPKYVQQ VQVSCFNELE VCIHPDGVIP VLTFLRDHTN AQFKSLVDLT AVDVPTRQNR FEIVYNLLSL RFNSRIRVKT YTDELTPIES AVSVFKAANW YEREIWDMFG VFFANHPDLR RILTDYGFEG HPFRKDFPLS GYVELRYDDE VKRVVAEPVE LAQEFRKFDL NSPWEAFPVY RQPPESLKLE AGDKKPDAK