Recombinant mouse Galectin-4/LGALS4 protein

Lgals4, also known as galectin4, belongs to a subfamily of galectins composed of two carbohydrate recognition domains within the same peptide chain. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions, which inhibits chronic inflammations, GVHD, and allergic reactions. The expression of this gene is restricted to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer. Recombinant mouse Lgals4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

The ED50 for this effect is equal or less than5 µg/mL. Measured by its ability to agglutinate human red blood cells.

Product Name Alternative

Galectin-4, Gal-4, Lactose-binding lectin 4

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

The ED50 for this effect is equal or less than 5 µg/mL. Measured by its ability to agglutinate human red blood cells.

Molecular Weight

38.8 kDa (349aa) confirmed by MALDI-TOF

Additionnal Information

Gal 4, Gal4, Gal-4, Galectin 4, Galectin4, Galectin-4, LGALS4, LGALS 4, ATGP1784-10 µg, ATGP1784-20 µg, ATGP1784-50 µg, ATGP1784-100 µg, ATGP1784-250 µg, ATGP1784-500 µg, ATGP1784-1 mg, ATGP1784-010, ATGP1784-020, ATGP1784-050, ATGP1784-100, ATGP1784-250, ATGP1784-500, ATGP1784-01M

References & Citations

Huflejt ME., et al. (1997) J Biol Chem. 272 (22) :14294-303. ; ; Huflejt ME., et al. (2004) Glycoconj J. 20 (4) :247-55.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_034836

Uniprot Accession Number

Q8K419

Species

Mouse

AA Sequence

MAYVPAP GYQPTYNPTL PYKRPIPGGL SVGMSVYIQG MAKENMRRFH VNFAVGQDDG ADVAFHFNPR FDGWDKVVFN TMQSGQWGKE EKKKSMPFQK GKHFELVFMV MPEHYKVVVN GNSFYEYGHR LPVQMVTHLQ VDGDLELQSI NFLGGQPAAA PYPGAMTIPA YPAGSPGYNP PQMNTLPVMT GPPVFNPRVP YVGALQGGLT VRRTIIIKGY VLPTARNFVI NFKVGSSGDI ALHLNPRIGD SVVRNSFMNG SWGAEERKVA YNPFGPGQFF DLSIRCGMDR FKVFANGQHL FDFSHRFQAF QMVDTLEING DITLSYVQI