Recombinant mouse Galectin-4/LGALS4 protein
Lgals4, also known as galectin4, belongs to a subfamily of galectins composed of two carbohydrate recognition domains within the same peptide chain. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions, which inhibits chronic inflammations, GVHD, and allergic reactions. The expression of this gene is restricted to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer. Recombinant mouse Lgals4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
The ED50 for this effect is equal or less than5 µg/mL. Measured by its ability to agglutinate human red blood cells.
Product Name Alternative
Galectin-4, Gal-4, Lactose-binding lectin 4
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
The ED50 for this effect is equal or less than 5 µg/mL. Measured by its ability to agglutinate human red blood cells.
Molecular Weight
38.8 kDa (349aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Huflejt ME., et al. (1997) J Biol Chem. 272 (22) :14294-303. ; ; Huflejt ME., et al. (2004) Glycoconj J. 20 (4) :247-55.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_034836
Uniprot Accession Number
Q8K419
Species
Mouse
AA Sequence
Frequently Asked Questions
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