Recombinant human MAGP-1/MFAP2 protein

Microfibrillar-associated protein 2, also known as MFAP2, is an O-glycosylated protein secreted to the extracellular space and the extracellular matrix. MFAP2 associates with biglycan and elastin in a ternary complex. It is shown to play a significant role in the support and distensibility of the juxtacanalicular region of these collector channels. It is also reported to inhibit LTB-1 binding to fibrillin-1, stimulate the phosphorylation of Smad2, and thereby mediate the subsequent extracellular deposition of latent TGFbeta. Recombinant human MFAP2, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

MAGP, MAGP-1, Microfibrillar-associated protein 2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

21.5 kDa (190aa)

Additionnal Information

MAGP, MAGP 1, MAGP1, MAGP-1, MFAP-2, MFAP 2, MFAP2, Microfibrillar-associated protein 2, ATGP1781-10 µg, ATGP1781-20 µg, ATGP1781-50 µg, ATGP1781-100 µg, ATGP1781-250 µg, ATGP1781-500 µg, ATGP1781-1 mg, ATGP1781-010, ATGP1781-020, ATGP1781-050, ATGP1781-100, ATGP1781-250, ATGP1781-500, ATGP1781-01M

References & Citations

Segade F., et al. (2002) J Biol Chem. 277:11050-11057.; ; Penner A S., et al. (2002) J Biol Chem. 277:35044-35049.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1M urea, 20% glycerol

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_002394.1

Uniprot Accession Number

P55001

Species

Human

AA Sequence

QGQYDL DPLPPFPDHV QYTHYSDQID NPDYYDYQEV TPRPSEEQFQ FQSQQQVQQE VIPAPTPEPG NAELEPTEPG PLDCREEQYP CTRLYSIHRP CKQCLNEVCF YSLRRVYVIN KEICVRTVCA HEELLRADLC RDKFSKCGVM ASSGLCQSVA ASCARSCGSC