Recombinant human NDUFAF1 protein

NDuFAF1 is a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The protein is required for assembly of complex I, and mutations in this protein are a cause of mitochondrial complex I deficiency. Recombinant human NDuFAF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

NADH:ubiquinone oxidoreductase complex assembly factor 1, Complex I intermediate-associated protein 30 mitochondrial, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1, CIA30, CGI-65

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

37 kDa (326aa) confirmed by MALDI-TOF

Additionnal Information

NDUFAF1, Complex I intermediate-associated protein 30, CGI-65; CGI65; CIA30, ATGP1775-10 µg, ATGP1775-20 µg, ATGP1775-50 µg, ATGP1775-100 µg, ATGP1775-250 µg, ATGP1775-500 µg, ATGP1775-1 mg, ATGP1775-10, ATGP1775-20, ATGP1775-50, ATGP1775-100, ATGP1775-250, ATGP1775-500, ATGP1775-1

References & Citations

Vogel RO., et al. (2005) . FEBS J. 272 (20) :5317-26.; ; Janssen R., et al. (2002) . Hum Genet. 110 (3) :264-70.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 1mM DTT, 2mM EDTA

Scientific Category

Metabolism

NCBI Accession Number

NP_057097

Uniprot Accession Number

Q9Y375

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSYPFLGIR FAEYSSSLQK PVASPGKASS QRKTEGDLQG DHQKEVALDI TSSEEKPDVS FDKAIRDEAI YHFRLLKDEI VDHWRGPEGH PLHEVLLEQA KVVWQFRGKE DLDKWTVTSD KTIGGRSEVF LKMGKNNQSA LLYGTLSSEA PQDGESTRSG YCAMISRIPR GAFERKMSYD WSQFNTLYLR VRGDGRPWMV NIKEDTDFFQ RTNQMYSYFM FTRGGPYWQE VKIPFSKFFF SNRGRIRDVQ HELPLDKISS IGFTLADKVD GPFFLEIDFI GVFTDPAHTE EFAYENSPEL NPRLFK