Recombinant human RNA polymerase II subunit RPB11-a/POLR2J protein
POLR2J is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This protein exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22. 1 and a pseudogene is found on chromosome 7p13. Recombinant human POLR2J protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
DNA-directed RNA polymerase II subunit RPB11-a, hRPB14, POLR2J1, RPB11, RPB11A, RPB11m
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
15.7 kDa (140aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Proshkin, S.A., et al. (2011) Biochemistry Mosc. 76 (8), 976-980; ; Shpakovskii, D.G., et al. (2004) Bioorg. Khim. 30 (6), 621-625
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 5mM DTT, and 2mM EDTA
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_006225
Uniprot Accession Number
P52435
Species
Human
AA Sequence
MNAPPAF ESFLLFEGEK KITINKDTKV PNACLFTINK EDHTLGNIIK SQLLKDPQVL FAGYKVPHPL EHKIIIRVQT TPDYSPQEAF TNAITDLISE LSLLEERFRV AIKDKQEGIE
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