Recombinant human RNA polymerase II subunit RPB11-a/POLR2J protein
POLR2J is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This protein exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22. 1 and a pseudogene is found on chromosome 7p13. Recombinant human POLR2J protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
DNA-directed RNA polymerase II subunit RPB11-a, hRPB14, POLR2J1, RPB11, RPB11A, RPB11m
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
15.7 kDa (140aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Proshkin, S.A., et al. (2011) Biochemistry Mosc. 76 (8), 976-980; ; Shpakovskii, D.G., et al. (2004) Bioorg. Khim. 30 (6), 621-625
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 5mM DTT, and 2mM EDTA
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_006225
Uniprot Accession Number
P52435
Species
Human
AA Sequence
MNAPPAF ESFLLFEGEK KITINKDTKV PNACLFTINK EDHTLGNIIK SQLLKDPQVL FAGYKVPHPL EHKIIIRVQT TPDYSPQEAF TNAITDLISE LSLLEERFRV AIKDKQEGIE
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
CD44 Standard (156-3C11), CF740 conjugate, 0.1mg/mL
CD50 (ICAM-3) (186-2G9), CF647 conjugate, 0.1mg/mL
CD44 Standard (156-3C11), CF488A conjugate, 0.1mg/mL
CD45RO (190-2F2.5), CF405S conjugate, 0.1mg/mL
