Recombinant mouse Galectin-8/LGALS8 protein

LGALS8, also known as prostate-specific membrane antigen (PCTA1), is an additional prostate-specific antigen that is overexpressed only in malignant tumors and therefore is a more specific identifier of malignancies. It is a member of the galectin gene family which mediates both cell-cell and cell matrix interactions in a manner similar to the selectin subgroup of C-type lectins. Recombinant mouse LGALS8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Measured by its ability to agglutinate human red blood cells. The ED50 range for this effect is ≤ 2 µg/mL.

Product Name Alternative

Lectin galactose binding soluble 8, Lgals-8

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured by its ability to agglutinate human red blood cells. The ED50 range for this effect is ≤ 2 µg/mL.

Molecular Weight

38.6 KDa (339aa) confirmed by MALDI-TOF

Additionnal Information

1200015E08Rik, AI326142, D13Ertd524e, galactose binding, Lectin, Lectin galactose binding soluble 8, LGALS8, Lgals-8, soluble 8, ATGP1772-10 µg, ATGP1772-20 µg, ATGP1772-50 µg, ATGP1772-100 µg, ATGP1772-250 µg, ATGP1772-500 µg, ATGP1772-1 mg, ATGP1772-010, ATGP1772-020, ATGP1772-050, ATGP1772-100, ATGP1772-250, ATGP1772-500, ATGP1772-01M

References & Citations

Kobayashi T., et al. (2010) Leukemia. 24 (4) :843-50.; ; Streetly MJ., et al. (2010) Blood. 115 (19) :3939-48.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol,1mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_061374

Uniprot Accession Number

Q9JL15

Species

Mouse

AA Sequence

MLSLNNL QNIIYNPIIP YVGTITEQLK PGSLIVIRGH VPKDSERFQV DFQLGNSLKP RADVAFHFNP RFKRSSCIVC NTLTQEKWGW EEITYDMPFR KEKSFEIVFM VLKNKFQVAV NGRHVLLYAH RISPEQIDTV GIYGKVNIHS IGFRFSSDLQ SMETSALGLT QINRENIQKP GKLQLSLPFE ARLNASMGPG RTVVIKGEVN TNARSFNVDL VAGKTRDIAL HLNPRLNVKA FVRNSFLQDA WGEEERNITC FPFSSGMYFE MIIYCDVREF KVAINGVHSL EYKHRFKDLS SIDTLSVDGD IRLLDVRSW