Recombinant human NCF4 protein

NCF4 is a cytosolic regulatory of the superoxide-producing phagocyte NADPH-oxidase, amulticomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integratedcatalytic core of the enzyme system. Recombinant human NCF4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Neutrophil cytosol factor 4, NCF, P40PHOX, SH3PXD4

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

41.1 kDa (359aa) confirmed by MALDI-TOF

Additionnal Information

NCF4, Neutrophil cytosol factor 4, NCF, P40PHOX, SH3PXD4, ATGP1769-10 µg, ATGP1769-20 µg, ATGP1769-50 µg, ATGP1769-100 µg, ATGP1769-250 µg, ATGP1769-500 µg, ATGP1769-1 mg, ATGP1769-10, ATGP1769-20, ATGP1769-50, ATGP1769-100, ATGP1769-250, ATGP1769-500, ATGP1769-1

References & Citations

Eglinton, T.W., et al. (2012) Am. J. Gastroenterol. 107 (4), 589-596

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_000622

Uniprot Accession Number

Q15080

Species

Human

AA Sequence

MAVAQQLRAE SDFEQLPDDV AISANIADIE EKRGFTSHFV FVIEVKTKGG SKYLIYRRYR QFHALQSKLE ERFGPDSKSS ALACTLPTLP AKVYVGVKQE IAEMRIPALN AYMKSLLSLP VWVLMDEDVR IFFYQSPYDS EQVPQALRRL RPRTRKVKSV SPQGNSVDRM AAPRAEALFD FTGNSKLELN FKAGDVIFLL SRINKDWLEG TVRGATGIFP LSFVKILKDF PEEDDPTNWL RCYYYEDTIS TIKDIAVEED LSSTPLLKDL LELTRREFQR EDIALNYRDA EGDLVRLLSD EDVALMVRQA RGLPSQKRLF PWKLHITQKD NYRVYNTMP