Recombinant human ASPRV1 protein
ASPRV1, also known as MuNO, SASP and ASAPase, is a protein that contains 1 peptidase A2 domain. This protein is expressed primarily in the granular layer of the epidermis and inner root sheath of hair follicles and localized to membrane region. This protein undergoes autocleavage which is necessary for activation of the protein. Recombinant human ASPRV1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Aspartic peptidase retroviral-like 1, Aspartic peptidase, retroviral-like 1, MuNO, SASP, SASPase, Taps
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
17.2 kDa (159aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Matsui, T., et al. (2011) EMBO Mol Med 3 (6), 320-333 ; ; Rhiemeier, V., et al. (2006) Am. J. Pathol. 168 (4), 1354-1364
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol,1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_690005
Uniprot Accession Number
Q53RT3
Species
Human
AA Sequence
SMGKGYY LKGKIGKVPV RFLVDSGAQV SVVHPNLWEE VTDGDLDTLQ PFENVVKVAN GAEMKILGVW DTAVSLGKLK LKAQFLVANA SAEEAIIGTD VLQDHNAILD FEHRTCTLKG KKFRLLPVGG SLEDEFDLE
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